Electrophoretic and Titrimetric Measurements on Bacterial Flagella.
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چکیده
منابع مشابه
Force-extension measurements on bacterial flagella: triggering polymorphic transformations.
Bacterial flagella can adopt several different helical shapes in response to varying environmental conditions. A geometric model by Calladine ascribes these discrete shape changes to cooperative transitions between two stable tertiary structures of the constituent protein, flagellin, and predicts an ordered set of 12 helical states called polymorphic forms. Using long polymers of purified flage...
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A wet-mount technique for staining bacterial flagella is highly successful when a stable stain and regular slides and cover slips are used. Although not producing a permanent mount, the technique is simple for routine use when the number and arrangement of flagella are critical in identifying species of motile bacteria.
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Many species of bacteria actively seek out favourable conditions for growth by swimming up gradients of nutrients, oxygen, light or other attractants, or down gradients away from toxic substances (repellants). Different species employ several different modes of swimming, almost all of which are driven by the rapid rotation of helical flagellar filaments that protrude from the cell. Rotation of ...
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was developed. This procedure involved measuring the binding of purified, radioactively labeled, antiflagellar antibodies to bacteria. The assay was used to determine the requirements for ribonucleic acid (RNA) and protein synthesis during flagellar regeneration in Bacillus subtilis. Immediate inhibition of flagella development was observed when chloramphenical or puromycin was added to cells. ...
متن کاملPeriodic chirality transformations propagating on bacterial flagella.
When a helical bacterial flagellum, clamped at one end, is placed in an external flow, it has been observed that regions of the flagellum transform to the opposite chirality, and travel as pulses down the length of the filament, the process repeating periodically [H. Hotani, J. Mol. Biol. 156, 791 (1982)]]. We propose a theory for this phenomenon based on a treatment of the flagellum as an elas...
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1950
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.04-0260